The aim of the present work was the characterization of nuclear bodies in the microspore and developing pollen cells of Hyacinthus orientalis L.. The combination of Ag-NOR, immunofluorescence and immunogold techniques was used in this study. The obtained results showed the presence of highly agyrophylic extranucleolar bodies in microspore and developing pollen cells, which were finally identifiedas Cajal bodies. In all cases, a strong accumulation of snRNP-indicating molecules including TMG cap, Smproteins and U2 snRNA, was observed in the examined nuclear bodies. In contrast to their number thesize of the identified structures did not change significantly during pollen development. In the microsporeand the vegetative cell of pollen grains CBs were more numerous than in the generative cell. At laterstages of pollen development, a drastic decrease in CB number was observed and, just before anthesis, acomplete lack of these structures was indicated in both pollen nuclei. On the basis of these results, as well as our previous studies, we postulate a strong relationship between Cajal body numbers and the levels ofRNA synthesis and splicing machinery elements in microspore and developing pollen cells.
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机译：本研究的目的是表征Hyacinthus Orientalis L.的小孢子和发育中的花粉细胞中的核小体。本研究使用了Ag-NOR，免疫荧光和免疫金技术的组合。获得的结果表明在小孢子和发育中的花粉细胞中存在高度糖基化的核仁外体，最终将其鉴定为Cajal体。在所有情况下，在被检查的核体内都观察到了包括TMG帽，Sm蛋白和U2 snRNA在内的snRNP指示分子的大量积累。与它们的数量相反，在花粉发育过程中，已鉴定结构的大小没有明显变化。在花粉粒的小孢子和营养细胞中，CB的数量比在生殖细胞中的要多。在花粉发育的后期，观察到CB数量急剧下降，并且在开花前，两个花粉核都表明这些结构完全缺乏。根据这些结果以及我们以前的研究，我们推测Cajal体数与小孢子和发育中的花粉细胞中RNA合成水平和剪接机械元件之间的密切关系。
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